D-LACTATE DEHYDROGENASE

Used for enzymatic determination of ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities.

:It's useful for enzymatic determination of numerous metabolites, e.g. ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g. GPT, PK when  coupled with the related enzymes.

Product Description
Appearance: White powder, lyophilized
Source: Microorganism
Enzyme Comission Number:EC 1.1.1.28
CAS Number: 9028-36-8
Storage Temperature -20C
Specific Activity: ≥ 400U/mg protein
Unit definition:One unit will convert one micromole of pyruvate to D-lactate per min at pH 7.4 at 25C.

Properties
Molecular Weight:38 kDa (SDS-PAGE)
Isoelectic point: 5.8
Michaelis constant: 5.4×10-4M
(Pyruvate, pH 7.0)
Optimum pH: 7.0
Optimum temperature: 30°C
pH Stability: 5.0~8.0 (25°C, 48hr)
Thermal Stability: < 45°C (pH7.0, 15min)
Inhibitors: Ag+,Hg2+

 Preparation Instructions
The enzyme is reconstituted in 100mM potassium phosphate buffer with 0.1% BSA, pH7.4 for activity assay.