GLUTAMATE DEHYDROGENASE

This enzyme catalyzes the reversible deamination of L-glutamate used for assay of NH3, ketoglutaric, L-glutamate and urease.

Glutamate Dehydrogenase catalyzes the reversible deamination of L-glutamate. It’s useful for enzymatic assay of NH3, α-ketoglutaric, L-glutamate and urease. It’s also can be used for enzymatic determination of urea when coupled with urease in clinical analysis.

 Product Description
    Appearance: White amorphous powder, lyophilized
    Source: Microorganism
    Enzyme Comission Number:EC 1.4.1.2
    CAS Number: 9001-46-1
    Storage Temperature -20°C
    Specific activity: ≥ 500U/mg protein
    Unit definition:One unit will convert one micromole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30°C.

Properties
    Molecular Weight: 260 kDa (gel)
    Isoelectic point: 5.6
    Michaelis constant:9.5×10-3M (NH3) 5.0×10-3M (α-Ketoglutarate) 8.4×10-5M (NADH)
    Optimum pH: 8.5(α-KG→L-Glu)
    Optimum temperature: 45°C
    pH Stability: 5.0~11.0
    Thermal Stability: < 60°C (pH8.3, 10min)
    Inhibitors: Ag+,Hg2+,Cu2+,Zn2+

Preparation Instructions
    The enzyme is reconstituted in 100mM Tris-HCl buffer, pH 8.3 for activity assay.