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GLYCEROL KINASE
this enzyme convert glycerol to glycerol-3-phosphate, used for enzymatic assay of glycerol and triglyceride coupled with enzymes
:Glycerol kinase can convert glycerol to glycerol-3-phosphate. It’s useful for enzymatic assay of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.
Product Description
Appearance: White powder, lyophilized
Source: Microorganism
Enzyme Comission Number:EC 2.7.1.30
CAS Number: 9030-66-4
Storage Temperature -20°C
Activity: ≥ 250U/mg protein
Unit definition:One unit will convert one micromole of glycerol to glycerol-3-phosphate per min at pH 7.9 at 37°C.
Properties
Molecular Weight: 55 kDa (SDS-PAGE)
Isoelectic point: 5.1
Michaelis constant: 3.8×10-5M (Glycerol)
Optimum pH: 8.0
Optimum temperature: 80°C
pH Stability: 5.0~10.0 (30°C, 20hr)
Thermal Stability: < 60°C (pH7.5, 30min)
Inhibitors: Ag+,Hg2+:
Preparation Instructions: The enzyme is reconstituted in 20mM potassium phosphate buffer, pH 7.5 for activity assay.
