- Pantyhose / Tights[4]
- Socks[10]
- Stockings[1]
- Catalyst[6]
- Chemical Auxiliary Agent[9]
- Food Additives[3]
- Other Lab Supplies[4]
- Organic Intermediate[9]
- Swelling[1]
- Pharmaceutical[1]
- Sweatband[6]
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3-alpha -HYDROXYSTEROID DEHYDROGENASE
It catalyzes the dehydrogenation of hydroxysteroid. It is useful for the determination of total bile acids in clinical analysis.
3alpha-Hydroxysteroid dehydrogenase catalyzes the dehydrogenation of hydroxysteroid. It is useful for the determination of total bile acids in clinical analysis.
Product Description
Appearance: White powder, lyophilized
Source: Microorganism
Enzyme Comission Number:EC 1.1.1.50
CAS Number: 9028-56-2
Storage Temperature -20°C
Specific activity: ≥ 50U/mg protein
Unit definition:One unit will oxidate one micromole of androsterone per min at pH 8.9 at 25°C.
Properties
Molecular Weight:28 kDa (SDS-PAGE)
Isoelectic point: 7.5
Michaelis constant: 3.0×10-5M(androsterone) 6.0×10-6M(NAD+)
Optimum pH: 10.5
Optimum temperature: 60°C
pH Stability: 4.5~10.5
Thermal Stability: < 50°C (pH7.2, 20min)
Inhibitors: Ag+,Hg2+
Preparation Instructions
The enzyme is reconstituted in 30mM potassium phosphate buffer, pH 7.2 for activity assay.
